twoBitToFa在UCSC下载小鼠的mm10版本基因组数据时没有找到.fa文件，发现了一个mm10.2bit文件，估计是把基因组序列存成了二进制文件，翻看文件说明：mm10.2bit - contains the complete mouse/mm10 genome sequence in the 2bit file format. Repeats from RepeatMasker a... The UCSC utility twoBitTofa  was used to obtain the. nucleotide sequences for the transcripts giving us a fastA file that was used for further compar-ative analysis. Long non-coding RNA I am trying to convert "hg19.2bit" to "hg19.fa" by the "twoBitToFa". I downloaded both files. I have "ubuntu" linux software. I used the following command line to convert the "2bit" to "fa" format as : "twoBitToFa hg19.2bit hg19.fa" but it says "twoBitToFa" is not recognized as a command. what sould I do to use "twoBitToFa" on linux. sincerely, mehmet.--Mehmet Ilyas COSACAK Undergraduate To complete the steps below you must first download the faToTwoBit, twoBitInfo, and twoBitToFa utilities. For more information on downloading our command-line utilities, see these instructions. To create a twoBit file, follow these steps: Prepare the sequence for your twoBit file in a FASTA-formatted file (i.e. genome.fa). the software dependencies will be automatically deployed into an isolated environment before execution.
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